ABSTRACT
Background: The quality of the archival samples stored at pathology services could be a limiting factor for molecular biology studies. Aim: To determine the quality of DNA extracted from gallbladder cancer samples at different institutions. Material and Methods: One hundred ninety four samples coming from fve medical centers in Chile, were analyzed. DNA extraction was quantifed determining genomic DNA concentration. The integrity of DNA was determined by polymerase chain reaction amplification of different length fragments of a constitutive gene (β-globin products of 110, 268 and 501 base pairs). Results: The mean DNA concentration obtained in 194 gallbladder cancer samples was 48 ± 43.1 ng/µl. In 22% of samples, no amplification was achieved despite obtaining a mean DNA concentration of 58.3 ng/ul. In 81, 67 and 22% of samples, a DNA amplification of at least 110, 268 or 501 base pairs was obtained, respectively. No differences in DNA concentration according to the source of the samples were demonstrated. However, there were marked differences in DNA integrity among participating centers. Samples from public hospitals were of lower quality than those from private clinics. Conclusions: Despite some limitations, in 80% of cases, the integrity of DNA in archival samples from pathology services in our country would allow the use of molecular biology techniques.
Subject(s)
Humans , DNA, Neoplasm/isolation & purification , Gallbladder Neoplasms/genetics , Chile , Cholecystectomy , DNA, Neoplasm/standards , Gallbladder Neoplasms/pathology , Nucleic Acid Amplification Techniques/methods , Pathology Department, Hospital , Polymerase Chain Reaction/methods , Quality Control , Sample SizeABSTRACT
Gallbladder cancer is a rare disease in Western developed countries, but it is a highly prevalent and lethal disease in Chile and other countries in Latin America. No randomized controlled trials have been performed in gallbladder cancer to establish standard treatments. We therefore performed the first Latin American consensus meeting for the management of gallbladder cancer. In this article we present the conclusions of the panel of experts for the palliative treatment of unresectable or metastatic gallbladder cancer based on a review of the literature, the discussion of the participating experts and the opinion of the assistants. The topics reviewed included: 1.- Gallbladder Cancer and Cholangiocarcinoma -are they the same disease?; 2. - Palliative Chemotherapy: Indications, Drugs and Schedules; 3. - Palliative Radiotherapy; 4.- Palliative Surgery; 5.-Management of Malignant Biliary Obstruction.
Subject(s)
Humans , Cholangiocarcinoma/drug therapy , Gallbladder Neoplasms/drug therapy , Palliative Care , Gallbladder Neoplasms/secondary , Latin America , Societies, MedicalABSTRACT
La fístula proveniente de la anastomosis esofagoyeyunal aún representa un aimportante causa de mortalidad después de la gastrectomía total. La presencia de compromiso pleural y la sepsis son comunes entre los pacientes que fallecen de una fñistula a este nivel. Reportamos dos casos de pacinetes con fístulas provenientes de la anastomosis esofagoyeyunal y compromiso pleural quienes fueron satisfactoriamente tratados. En ambos pacientes, el manejo agresivo consistió en aislamiento de la anastomosis del contenido esofágico. Este procedimiento fue esencial en la recuperación de los pacientes